Dog walkers are being told they must put their pets on a lead if visiting the Ayres National Nature Reserve.
It's in order to protect ground nesting birds – the restriction is in place between April and July.
People who don’t adhere to the rule could be fined up to ?2,500.
Curlew, lapwing, oystercatcher, ringed plover, little and Arctic tern, skylark and meadow pipit all lay eggs on the ground at the reserve.
When chicks hatch, they move away from nests under the watchful eye of their parents. It can be five weeks before they can fly, during which time they are vulnerable.
Visitors to the reserve are being asked to keep to the main paths and move away if birds show signs of agitation or distress.
Environment, Food and Agriculture Minister Geoffrey Boot says: "The Ayres and its birdlife are very important to the balance and diversity of wildlife in the Isle of Man.
"It has a unique environment which is not found anywhere else on the Island and attracts birds that are both rare and declining in numbers.
"We must all be custodians of our unique biosphere which has been recognised by UNESCO.
"We appeal to dog owners to act responsibly and we will be working with the Isle of Man Constabulary to ensure anyone who flouts the bylaws is penalised."